Eltrombopag maintains human HSPCs in the presence of IFN-γ in vitro. (A) Schematic of the experimental design. (B) Representative flow cytometry plots of CD34 expression obtained after 7 days of culture. (C) Absolute CD34⁺ cell counts after 7 days of culture (n = 13, from 11 independent donors). (D) Normalized numbers of CFU derived from the 7-day cultures (n = 3 independent donors). (E) Human cell engraftment, as measured by human CD45-expressing cells in the BM of recipient NSG mice 12 weeks after transplantation of human CD34⁺ cells cultured for 7 days (n = 4-5 mice per group). Human lymphoid (CD20⁺) and myeloid (CD13⁺) cells were detected in all animals. (C-E) Data are mean ± standard error of the mean (SEM). **P < .01, ***P < .001, multiple 2-tailed unpaired Student t tests. ns, nonsignificant.