Formation of chemoattractant-induced pseudopodia in WT and PTENα^−/− neutrophils. (A) Visualization of formation of pseudopodia and changes in cellular morphology in isolated neutrophils after fMLP stimulation. Cells were stained with phalloidin (F-actin stain) and DAPI (nuclear stain). The images were acquired using a Nikon TCS A1 confocal microscope (100× lens objective). (B) The numbers of WT and PTENα^−/− neutrophil pseudopodia (n = 50) were counted after fMLP stimulation. (C) Analysis of the aspect ratio (ratio of the longest axis to the shortest perpendicular axis) of WT and PTENα^−/− neutrophils (n = 50) after fMLP stimulation. Data are mean ± standard error of the mean. ****P < .0001, 2-tailed unpaired Student t test.