Polarization is decreased in stimulated PTENα^−/− neutrophils. (A) Increased numbers of WT neutrophils showed visible polarization after MIP-2 stimulation. Cells were stained with phalloidin (F-actin stain) and DAPI (nuclear stain). The images acquired using a Nikon TCS A1 confocal microscope (100× lens objective). (B) The percentages of polarized WT (n = 133) and PTENα^−/− (n = 93) neutrophils (F-actin localizing at the leading edge of the cell) were recorded after MIP-2 stimulation.