Disruption of PTENα impairs host immune defense against S typhimurium infection. (A) Mouse survival curves post–S typhimurium infection. WT mice (n = 9) and PTENα^−/− mice (n = 8) were inoculated intraperitoneally with uniform amounts of bacteria. *P < .05, Mantel-Cox test. (B) Absolute number of neutrophils in elicited peritoneal cells with sham treatment or after intraperitoneal injection of S typhimurium (S.t.). BD Trucount Absolute count tubes were used to count absolute cell numbers. Data represent ≥6 mice derived from 3 independent experiments in each group (mean ± standard error of the mean). ***P < .001, 2-way repeated-measures ANOVA, followed by the Bonferroni post test. (C) Viable bacterial CFU in lungs of mice postinfection. Data are representative of 3 independent experiments with a total of 6 or 7 mice per group (mean ± standard error of the mean). *P < .05, 2-tailed unpaired Student t test. ns, not significant (P > .05).