Partial rescue of Trib1-deficient eosinophil mobilization in response to type 2 lung inflammation. Mice were treated for 5 days with intranasal PBS or 30 μg of papain. (A) Representative plots of lung leukocytes gated on live, CD45⁺CD11b^hiCD11c⁻. Frequency of live cells (B) and absolute number (C) of lung eosinophils gated on live, CD45⁺CD11b^hiCD11c⁻SiglecF⁺ cells. (D) Representative histogram of Ly6G expression by lung eosinophils gated live, CD45⁺CD11b^hiCD11c⁻SiglecF⁺, Trib1^+/+ (blue), Trib1^ΔEos (red). (E) Fraction of lung eosinophils expressing Ly6G; n = 3 mice per group, representative of 2 experiments. Frequencies and error bars are mean ± SEM of live cells.