Genomic region 6 kb downstream of Cebpe TSS has properties of an enhancer. (A) Publically available DNase I hypersensitive sites sequencing data for 416B myeloid cells, B lymphocytes, and T cells show that a region +6 kb from Cebpe TSS (region encompassing the +6-kb enhancer has been highlighted) is an open chromatin specifically in myeloid cells.²⁵  (B) Comparison of H3K4me1, H3K27ac, and H3K4me3 ChIP-seq signal in murine long-term hematopoietic stem cells (LT-HSC), B cells, common myeloid progenitors (CMP), granulocyte monocyte progenitors (GMP), and granulocytes (Gr).²⁶  (C) IGV tracks depict P300 ChIP-seq in 416B myeloid cells, B cells, and a hematopoietic progenitor cell line (HPC-7).^28-30  (D) +6-kb region is enclosed within a sub-TAD (shaded area), marked by CTCF and Rad21 binding in in vitro–differentiated ECOMG cells.³¹  (E) 4C-seq profile of murine bone marrow cells reveals strong interaction of the +6-kb enhancer with a region encompassing the Cebpe promoter (chr14: 55 330 487-55 335 487 mm9). Viewpoint region is the shaded area. Circles represent significant interactions detected in our 4C-seq experiments.