TNFα enhances the genomic binding of PHF6. (A) Pie chart showing the distribution of PHF6 and NF-κB (p65) peaks. (B) Venn diagram showing the overlap between PHF6 and NF-κB (p65) peaks. (C) Gene tracks showing PHF6 and NF-κB (p65) peaks in control and TNFα-treated K562 cells at the METRNL locus. The x-axis indicates the linear sequence of genomic DNA, and the y-axis represents the total number of mapped reads. (D) Box-and-whisker plots showing NF-κB (p65) and PHF6 ChIP combined with high-throughput sequencing signals in the NF-κB–bound regions identified in TNFα-treated K562 cells. Boxes represent 25 to 75 percentile ranges. Vertical lines represent 10 to 90 percentile ranges. Horizontal bars represent medians. Mean values are indicated by red crosses. (E) Gene tracks showing PHF6 and NF-κB (p65) peaks in control and TNFα-treated K562 cells at the NR4A1 locus. The x-axis indicates the linear sequence of genomic DNA, and the y-axis represents the total number of mapped reads. The region detected by ChIP qPCR is indicated by a red bar. (F) ChIP qPCR assays for NF-κB (p65) and PHF6 in control and TNFα-treated K562 cells at the NR4A1 locus. The relative amounts of immunoprecipitated DNA are depicted as percentages of input DNA. Data are shown as the mean plus or minus SEM of triplicate PCRs. *P < .05, **P < .01, ***P < .001 by the Student t test. NA, not applicable; n.s., not significant.