Increased fibrin deposition and evidence of RBC sickling in thrombi formed in the femoral vein of SCD mice. (A) Representative images of platelet (green) and fibrin (red) deposition within thrombi formed in the femoral vein of AA, AS, and SS mice 60 minutes after electrolytic injury. Yellow color shows overlap between fibrin and platelets. Representative movies demonstrating the dynamics of clot formation over 1 hour are included as supplemental Videos 2-4 for AA, AS, and SS mice, respectively. Intravital microscopy analysis of platelet accumulation (B) and fibrin deposition (C) within thrombi of AA (n = 14, gray line), AS (n = 9, purple line), and SS (n = 10, blue line) mice quantitated as a relative intensity. *P < .05. (D) Hematoxylin and eosin staining of the thrombus (delineated by black dotted line) formed in the femoral vein of SS mice demonstrates the presence of RBCs (dark pink) and fibrin/platelets (light pink). Extensive sickling of the RBCs is present within the thrombus (E) but not in the blood pool outside the thrombus (F). Original magnification: ×4 (D) and ×40 (E-F). NS, not significant.