Figure 2.
![Figure 2. Multiplex immunofluorescence. Comparison of BL and PP biopsy for patient with available tissue. (A) Increased PD-L1 on HRS (PAX5+, red arrows) cells and macrophages (CD68+). White arrow indicates PAX5+ B cell. Original magnification ×200; inset, original magnification ×200. (B) Increase in PD-1 on infiltrating T cells (CD3+). Original magnification ×200; inset, original magnification ×200. Stains: DAPI (Invitrogen); PDL1: 9A11 (Dana-Farber Cancer Institute [DFCI]/Cell Signaling Technology [CST]), Opal 520; PD1: EH33 (DFCI/CST), Opal 690; CD3: PolyC (Dako), Opal 570; CD68: PGM1 (Dako), Opal 540; PAX5: 24/PAX-5 (BD Transduction), Opal 620.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/134/1/10.1182_blood.2019000215/3/bloodbld2019000215f2.png?Expires=1735587872&Signature=pFMjFGzn~JvNaFwEd20c6IMQuJk~QOW70OmYLp40Rh4KALlbDTfnLju~mTuKA~6sH3gzRkKuZFGZvmN-ZW5h9Fgr2ypPtIALy1-ugNCLtwHMRjf2r78jZ1w9O-5V3WEZ0l82CoduPKvltiEIIv4Gy5wU3QThgGllqxKjBN6CueA3t1qjB54kKtLy9ju2Id3N446x9BXfzckEjhzGiJFRW4RduGjJkvImNpa1~h9brl4L6QWVgOP2k~aD68011bfdWaIDsaOVacu5kuxLw5VyO5NQijEL5YwAVjgZ88JXunIaSxv7DxLF0dcOfCdNR0lGPe9IenQgQSdrxTvMcjN7Bg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Multiplex immunofluorescence. Comparison of BL and PP biopsy for patient with available tissue. (A) Increased PD-L1 on HRS (PAX5+, red arrows) cells and macrophages (CD68+). White arrow indicates PAX5+ B cell. Original magnification ×200; inset, original magnification ×200. (B) Increase in PD-1 on infiltrating T cells (CD3+). Original magnification ×200; inset, original magnification ×200. Stains: DAPI (Invitrogen); PDL1: 9A11 (Dana-Farber Cancer Institute [DFCI]/Cell Signaling Technology [CST]), Opal 520; PD1: EH33 (DFCI/CST), Opal 690; CD3: PolyC (Dako), Opal 570; CD68: PGM1 (Dako), Opal 540; PAX5: 24/PAX-5 (BD Transduction), Opal 620.