Dormant myeloma cells express a distinct gene signature. scRNAseq analysis of eGFP⁺DiD^hi dormant and eGFP⁺DiD^neg reactivated cell populations using SMART-seq. (A) Heatmap of 1492 differentially expressed genes between dormant and reactivated cells. (B) Biological processes associated with the genes upregulated in dormant (D; gold) and upregulated in reactivated (R; gray) populations. (C) Consensus matrix of nonnegative matrix factorization analysis of 1492 differentially expressed genes between dormant and reactivated cells defines 2 dormant (gold; C1 and C2) and 1 reactivated (gray; C3) cluster. (D) Distribution of individual cell sample contributions to each of the 3 metagenes. (E) Distribution of all the genes (top) and the top 5 unique genes (bottom) contributing to each metagene. (F) Top 50 genes from the primary (C1) dormant metagene sorted by descending contribution. (G) Genes predicted to be controlled by the Irf7 and Spic transcription factors.