LPS or Mrp-8 activates TLR-4 and downstream NF-κB translocation into the nucleus; in the nucleus, NF-κB induces the synthesis of TNF-α and IL-10. Secondary stimulation with LPS activates PI3K/AKT and phosphorylates GSK3β; inactive phosphorylated GSK3β does not degrade IκBα and impairs NF-κB activation. IL-10, via IL-10R, phosphorylates STAT3 that induces BCL-3 synthesis. BCL-3 promotes binding of inactive NF-κB to the TNF promoter. R, receptor