PD-L1 CRISPR library screen in ALK⁺ ALCL. (A) Outline of the workflow of the PD-L1 CRISPR library screen in ALK⁺ ALCL. (B) Overview of the PD-L1 CRISPR library screen results in ALK⁺ ALCL (2 repeats). Shown are results of all sgRNA enrichment in PD-L1^low population [measured by log2(PD-L1^low/unsorted)] in DEL line. (C) Shown are the enrichment score [measured by log²(PD-L1^low/unsorted)] of the top 10 sgRNAs identified in the PD-L1 CRISPR library screen of DEL line, in an average of 2 repeats. The sgCTRL score represents the average of 100 negative control sgRNAs. (D) Shown are the ranking of all the genes (average of 10 sgRNAs of each gene) enriched in the PD-L1^low population of both DEL and Karpas299 lines. Y-axis indicates the distribution of standardized enrichment scores (Z-scores) of each gene enrichment. The dashed lines indicate P = .01. (E) List of top 10 genes enriched in the PD-L1^low population of both DEL and Karpas299 lines. Y-axis indicates the distribution of standardized enrichment scores (Z-scores) of each gene enrichment. Green, genes common in both DEL and Karpas299 lines; red, genes in RAS-MEK-ERK pathway; blue, genes in the PI3K-AKT-mTOR pathway.