Sex differences in AR expression on neutrophils. ARs were detected in neutrophils from patients with DADA2. (A,D) Confocal analysis shows the presence of A₁AR and A₃AR (in red) in the plasma membrane of DADA2 neutrophils. Nuclei were stained in blue. Scale bar, 5 µm. (B) A₁AR was analyzed in neutrophils from patients with DADA2 by flow cytometry. Three populations of A₁AR were found in neutrophils from patients with DADA2. (C) Percentage of neutrophils with surface A₁AR, classified as negative, low, and high. (E) A₃AR was analyzed in neutrophils from patients with DADA2 by flow cytometry. (F) Percentage of neutrophil for A₃AR. (G) A₁AR and (H) A₃AR were analyzed in neutrophils from healthy with by flow cytometry. *P < .05 Mann-Whitney U test.