Figure 1.
Identification of Epor+ macrophages in mouse BM and FL using the Epor-eGFPcre knockin mouse model. (A) Flow cytometry analysis of mouse BM F4/80+Epor-eGFP+ macrophages. Gating procedures are described in supplemental Figure 1A. WT mouse BM cells were used as control. N = 6. (B) Histogram of Epor-eGFP (a), F4/80 (b), and Ly6G/Ter119 (c) of the sorted BM F4/80+Epor-eGFP+ and F4/80+Epor-eGFP− macrophages. Dotted line: Fluorescence Minus One control. (C) The composite cytospin images of the sorted BM F4/80+Epor-eGFP+ and F4/80+Epor-eGFP− macrophages. (D) Flowcytometry analysis of mouse FL F4/80+Epor-eGFP+ macrophages. WT mouse FL cells were used as control. N = 4. (E) Histogram of Epor-eGFP (a), F4/80 (b), and Ly6G/Ter119 (c) of the sorted FL F4/80+Epor-eGFP+ and F4/80+Epor-eGFP− macrophages. (F) The composite cytospin images of the sorted FL F4/80+Epor-eGFP+ and F4/80+Epor-eGFP− macrophages. FSC, forward scatter area; SSC, side scatter; WT, wild-type.