Figure 5.
Figure 5. Effects of Epo on EBIs and EBI macrophages in vivo. (A) EBI numbers in mouse BM with and without Epo. (B) Percentages of EBIs with 3, 4, and 5 or more erythroblasts with and without Epo. (C) Erythroblast numbers in mouse BM with and without Epo. (D) F4/80+Epor-eGFP+ macrophage numbers in mouse BM with and without Epo. (Ea) Representative Vcam1 expression profiles with and without Epo; (Eb) quantitative analyses of Vcam1 expression on mouse BM F4/80+Epor-eGFP+ macrophages with and without Epo. (Fa) Representative CD169 expression profiles with and without Epo; (Fb) quantitative analyses of CD169 expression on mouse BM F4/80+Epor-eGFP+ macrophages with and without Epo. (Ga) Representative CD163 expression profiles with and without Epo; (Gb) quantitative analyses of CD163 expression on mouse BM F4/80+Epor-eGFP+ macrophages with and without Epo; (Gc) the percentage of CD163+ cells within BM F4/80+Epor-eGFP+ macrophages with and without Epo. *P < .05, **P < .01, ***P < .001. N = 3.

Effects of Epo on EBIs and EBI macrophages in vivo. (A) EBI numbers in mouse BM with and without Epo. (B) Percentages of EBIs with 3, 4, and 5 or more erythroblasts with and without Epo. (C) Erythroblast numbers in mouse BM with and without Epo. (D) F4/80+Epor-eGFP+ macrophage numbers in mouse BM with and without Epo. (Ea) Representative Vcam1 expression profiles with and without Epo; (Eb) quantitative analyses of Vcam1 expression on mouse BM F4/80+Epor-eGFP+ macrophages with and without Epo. (Fa) Representative CD169 expression profiles with and without Epo; (Fb) quantitative analyses of CD169 expression on mouse BM F4/80+Epor-eGFP+ macrophages with and without Epo. (Ga) Representative CD163 expression profiles with and without Epo; (Gb) quantitative analyses of CD163 expression on mouse BM F4/80+Epor-eGFP+ macrophages with and without Epo; (Gc) the percentage of CD163+ cells within BM F4/80+Epor-eGFP+ macrophages with and without Epo. *P < .05, **P < .01, ***P < .001. N = 3.

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