Figure 5.
Effects of Epo on EBIs and EBI macrophages in vivo. (A) EBI numbers in mouse BM with and without Epo. (B) Percentages of EBIs with 3, 4, and 5 or more erythroblasts with and without Epo. (C) Erythroblast numbers in mouse BM with and without Epo. (D) F4/80+Epor-eGFP+ macrophage numbers in mouse BM with and without Epo. (Ea) Representative Vcam1 expression profiles with and without Epo; (Eb) quantitative analyses of Vcam1 expression on mouse BM F4/80+Epor-eGFP+ macrophages with and without Epo. (Fa) Representative CD169 expression profiles with and without Epo; (Fb) quantitative analyses of CD169 expression on mouse BM F4/80+Epor-eGFP+ macrophages with and without Epo. (Ga) Representative CD163 expression profiles with and without Epo; (Gb) quantitative analyses of CD163 expression on mouse BM F4/80+Epor-eGFP+ macrophages with and without Epo; (Gc) the percentage of CD163+ cells within BM F4/80+Epor-eGFP+ macrophages with and without Epo. *P < .05, **P < .01, ***P < .001. N = 3.