Findings in patients with CLL clone ≥0.5 × 10⁹/L (N = 30). (A) Mutational profile, by NGS. Each column represents a patient. BTK mutations had no association with TP53 mutations or other mutations. PLCG2 mutations were all associated with BTK mutations and TP53 mutations. (B) Outcomes subsequent to sample collection in presence (n = 17) or absence (n = 13) of BTK mutations, with regard to CLL progression and treatment. Each bar represents a patient. IGHV, immunoglobulin heavy chain variable gene; ND, not determined.