In vivo neutralization of TNF-α decreases platelet hyperreactivity and restores mitochondrial mass in aged mice. (A) Experimental approach. (B) Decreased plasma levels of TNF-⍺ in old mice treated with anti–TNF-⍺ antibody (n = 4-5 mice per group; mean plus or minus SEM). (C) Platelet counts did not significantly change between old mice treated with anti–TNF-⍺–neutralizing antibodies or isotype control (n = 5 mice per group; mean plus or minus SEM). (D) Inhibition of the ⍺_IIbβ₃ integrin by anti–TNF-⍺–neutralizing antibodies as measured by FACS (n = 4 mice per group; mean plus or minus SEM). (E) Platelets from old mice treated with by anti–TNF-⍺–neutralizing antibodies show significantly decreased platelet adhesion over collagen-coated slides under flow at 650 s⁻¹ (n = 4-5 mice per group; mean plus or minus SEM). (F) Mitochondrial mass assessment after anti–TNF-⍺–neutralizing antibodies (n = 3-4 mice per group; mean plus or minus SEM). *P < .05; **P < .01; and ***P < .001. Student t test and ANOVA.