Hierarchy of TP53 alterations in myeloma. (A) Experimental approach to produce mono- and biallelic TP53 fluorescence-tagged clones. (B) Clonal competition assays of TP53 biallelic (red), TP53 monoallelic (yellow), and TP53 WT AMO1 cells (purple). Top-row experiments were performed in triplicate. The initial percentage of the subclone(s) at day 0 and at the end of the experiment is included in each figure. The share of the different clones based on the fluorescence signals of enhanced green fluorescent protein and/or LSS-Kate2 red fluorescent protein was determined every 4 to 14 days (black dots/bars). Error bars for the top-row figures represent the standard deviation based on 3 independent experiments.