Clonal architecture of different pathway mutations. (A) Mean ± SEM of VAFs of common driver mutations in the cohort. (B) Dot plots depict VAFs of pairwise co-occurring mutations. Gene mutations with higher VAFs are considered to occur earlier then variants with lower VAFs. Dark gray square highlights 45% VAF. Dots appearing in the dark gray square are possibly acquired as subclones. Dot plot shows different mutation acquisition orders of SRSF2, ASXL1, and signaling molecules (C) and TET2, EZH2, and signaling molecules (D).