PGN induces TF expression in primary human monocytes in vitro. (A) Exemplification of PBMC procoagulant responses induced by stimulation with either PGN (10 µg/mL) or LPS (1 µg/mL) for 6 hours, as identified by TF expression in CD14⁺ monocytes in a median responsive individual. (B) Histogram overlay of TF expression in CD14⁺ PBMCs after PAMP stimulation of a median responsive individual. (C) PAMP induction of procoagulant monocytes (TF⁺CD14⁺) in the human cohort studied (n = 86 independent measurements). Horizontal bars depict median and IQR, and outliers are identified by red symbols. (D) Monocyte TF antigen expression (geometric mean of fluorescence intensity) in PAMP-stimulated PBMCs (n = 86). Median, IQR, and outlier representation is consistent with panel C. (E) Correlation between LPS- and PGN-induced procoagulant responses in the study cohort. Individual data represent paired, log-normalized, TF fluorescence intensities of LPS- or PGN-stimulated PBMCs assessed in parallel. Best-fit linear regression (continuous line) and 95% confidence intervals (dotted lines) are graphically represented, and Pearson correlation coefficient (r) is shown in inset. APC, allophycocyanin.