PGN induces monocyte TF expression in a dose- and time-dependent manner. (A) PGN induces monocyte TF expression in a dose-dependent manner. Representative overlaid histograms of a median responsive individual (left) and average (n = 8 independent donors) monocyte procoagulant responses after 6 hours stimulation with PGN (0-50 µg/mL; middle and right panels). Procoagulant responses were measured in the presence of autologous serum at either high (10%; blue squares) or low (1%; red circles) concentration. Best-fit 4-parameter logistic curves used to estimate EC50 are depicted by continuous or dotted lines, respectively. (B) PGN (10 µg/mL) induces monocyte TF expression in a time-dependent manner. Representative overlaid histograms of a median responsive individual (left) and distribution of TF procoagulant responses (middle and right panels) in the cohort studied (n = 14). Individual time-course responses are identified by identical symbols, and horizontal bars indicate median and IQR of procoagulant responses to PGN in the cohort studied. RFI, relative fluorescence intensity.