161519 TriKE induces stronger NK cell–mediated killing of CLL patient tumor cells. Enriched allogeneic NK cells from healthy donors were incubated with noted molecules (30 nM for everything but IL-15 [used at 0.3 nM]) for 18 hours, washed, and then cocultured with CellTrace-labeled CLL targets in the presence of fresh noted treatments for a 4-hour period (n = 17; 2 experiments with 2-3 NK donors against 3-4 CLL targets). After the incubation period, CLL tumor cells were identified as CellTrace⁺/CD19⁺/CD5⁺ cells. Flow chart outlines the gating schema used to measure live target CLL cell percentage. Killing percentage was then calculated from live target cell percentage. NT denotes NK cells incubated with CLL targets without any molecules added and is used as a baseline of killing. One-way ANOVA with repeated measures was used to calculate differences against the 161519 group. Error bars indicate the mean ± standard error of the mean. Statistical significance are determined as ****P < .0001.