Metabolomics analysis of blood components post-storage and autologous transfusion. (A) Overview of the experimental design. Briefly, 18 healthy volunteers donated a unit of blood. Packed RBCs were thus split and stored for 5 and 42 days, respectively, prior to autologous reinfusion via brachial artery. (B) A heat map summarizing the main metabolic changes in the supernatants of units stored for 5 (top) and 42 days (bottom). Color codes indicate row minimum (min; blue) to maximum (max; red) upon z-score normalization across values measured in all samples. FA, fatty acids.