Figure 2.
![Figure 2. hbbe1.1 and alas2 are expressed in hmgcs1 mutant larvae. Whole mount ISH was performed to detect the expression of hbbe1.1 (n = 41 hmgcs1+/+ [sibling], n = 34 hmgcs1−/− [Vu57]), (A-B) or alas2 (n = 16 hmgcs1+/+ [sibling], n = 15 hmgcs1−/− [Vu57]), (C-D) at 26 hpf. Purple, expression of each gene in the ICM; arrowhead, areas of increased or abnormal expression. Total number of animals was achieved with a minimum of 2 biological replicates. Images were taken with a 10× optical lens at 6.3× objective zoom.](https://ash.silverchair-cdn.com/ash/content_public/journal/bloodadvances/3/8/10.1182_bloodadvances.2018024539/2/advances024539f2.png?Expires=1735573168&Signature=tfd~0CWJdhJwMs5698iStioTUNtI88eoLPO6SGTfXQzXg58vz7h1owMKs7olwfUDlNKfd3VAtu9GCVWXnV3OL2R3xlJCPQotpgwjIEgmRjYA8kAMcxDiukdQO49ovr0T-O0oXAO16IZ~Fd3elw4Y-E5bnTehxN3rEL7jCR94UkmUDmWjGk~ssZnZJwNTDIzb1Mjjkk7P~cuL3tbSVPbj1Kur7M~XNNPOqYShGZLdzOn33t0g3NLobTJ-uCAe-V5j~UFE3xM8STfV2ABG1jQKTb3e8M49iZNMqk8zVFmZj6Ig7SnInugbCr9C1ykttTmYWv3YlLuEozJaVWXe5AQ4BQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
hbbe1.1 and alas2 are expressed in hmgcs1 mutant larvae. Whole mount ISH was performed to detect the expression of hbbe1.1 (n = 41 hmgcs1+/+ [sibling], n = 34 hmgcs1−/− [Vu57]), (A-B) or alas2 (n = 16 hmgcs1+/+ [sibling], n = 15 hmgcs1−/− [Vu57]), (C-D) at 26 hpf. Purple, expression of each gene in the ICM; arrowhead, areas of increased or abnormal expression. Total number of animals was achieved with a minimum of 2 biological replicates. Images were taken with a 10× optical lens at 6.3× objective zoom.