Activation of α_IIbβ₃and P-selectin surface expression are not influenced by Nox2 deficiency in male or female mice. Activation of α_IIbβ₃ (detected by JON/A binding) (A-D) and surface expression of P-selectin (E-H) were measured by flow cytometry in washed platelets from male and female WT (+/y or +/+) or Nox2-deficient (−/y or −/−) mice following activation with convulxin or thrombin. Data are presented as mean ± standard error (n = 5 or 6 mice in each group). *P < .05 vs. WT (+/y or +/+) without convulxin/thrombin, ^$P < .05 vs. Nox2-deficient (−/y or −/−) without convulxin/thrombin, ^@P < .05 vs. WT (+/y or +/+) with convulxin (25 ng/mL)/thrombin (0.02 U/mL), ^#P < .05 vs. Nox2-deficient (−/y or −/−) with convulxin (25 ng/mL)/thrombin (0.02 U/mL), 2-way ANOVA with Tukey test for multiple comparisons.