Comparison ofthe classical pathway–mediated hemolysis between the C3-sufficient and C3-Dpl human or C3-deficient rat sera. For the measurements of classical pathway–mediated hemolysis, antibody-sensitized E^shA cells were incubated with different concentrations of NHS, C3-Dpl human sera (A,C), or WT C3-deficient (C3KO) rat sera (B,D) in GVB⁺⁺ buffer, and hemolysis was quantitated by measuring levels of released hemoglobin in the supernatants. These data show significantly reduced classical pathway–mediated hemolysis in the absence of C3 when lower concentrations of sera were used (A-B), but comparable and almost complete hemolysis when higher concentrations of sera were incubated (C-D). *P < .05