Mutation burden at diagnosis and relapse. DNA was extracted from primary, remission, and relapse samples and subjected to WES. The remission sample was used as a baseline control to exclude germline variants. (A) SNVs were quantified, and TMB was calculated, showing that the primary sample contained 9.4 mutations per megabase of DNA, whereas the relapse sample had 19.6 mutations per megabase. (B) A total of 457 SNVs was detected in the primary sample, and 953 SNVs were detected in the relapse sample. Of these, 98 SNVs were common to the primary and the relapse samples.