Bone marrow mononuclear cell numbers in patients with MM before and after cyclophosphamide treatment. (A) Absolute numbers of the 3 major bone marrow subtypes were quantified by using flow cytometry in bone marrow samples from 10 adults diagnosed with MM before (pretreatment) and 24 hours after (posttreatment) with CyBorD DARA. (B) Specific CD45⁺ bone marrow subpopulations were identified based on side scatter characteristics and surface expression of CD14. Representative gating strategy for bone marrow aspirates for the identification and quantification of lymphocytes, neutrophils, and monocytes. (C) Graphed dot plots indicate the absolute number of lymphocytes, neutrophils, and monocytes from bone marrow aspirates before (prescreened) and 24 hours’ posttreatment with CyBorD DARA (n = 10). (D) Dot plots indicate the absolute number of CD4⁺ and CD8⁺ T cells and NK cells pretreatment and posttreatment (n = 6). Gating for subpopulation of lymphocytes is shown in supplemental Figure 1. Lines between dots indicate paired samples. Wilcoxon matched pairs signed rank test and paired Student t tests were used to detect statistically significant differences between pretreatment and posttreatment samples. *P < .05, **P < .01. FSC-A, forward scatter–area; ns, not significant; SSC-A, side scatter–area.