Analysis of circulating proinflammatory and anti-inflammatory cytokines in MM patient serum before and after treatment with CyBorD DARA. (A) Representative scheme to illustrate the work flow for isolation and analysis of patient serum before and after treatment. (B) Dot plots indicate the levels of TNF-α (upper left, n = 12), IFN-γ (upper right, n = 12), interleukin-10 (IL-10) (lower left, n = 12), and PGE₂ (lower right, n = 12) before and after treatment in MM serum samples. Lines between dots indicate paired samples. Paired Student t tests were used to detect statistically significant differences between pretreatment and posttreatment samples. *P < .05. ELISA, enzyme-linked immunosorbent assay.