TF binding profile and consensus binding motifs for HCK promoter. (A) Findings from Promoter-binding studies to delineate TFs bound to the HCK promoter in MYD88-mutated cells. Heat maps indicate change in relative luminescence units (RLU%), which depict free unbound activated TFs in nuclear extracts from MYD88-mutated BCWM.1 cells. Top panel depicts RLU changes following the addition of HCK promoter sequence to nuclear extract of unstimulated BCWM.1 cells (BCWM.1 + HCK promoter vs BCWM.1). Middle panel depicts RLU changes in BCWM.1 cell nuclear extracts following LPS stimulation (BCWM.1 + LPS). Bottom panel depicts RLU changes in LPS-stimulated BCWM.1 cell nuclear extract following the addition of HCK promoter sequence (BCWM.1 + LPS + HCK promoter vs BCWM.1 + LPS). Blue to brown spectrum in heat maps indicates relative level increase in free activated TFs detected by Promoter-binding assay. (B) Cross-sample comparison of RLU% depicting free unbound activated TFs after addition of HCK promoter in unstimulated BCWM.1 cell nuclear extracts; LPS-stimulated BCWM.1 cell nuclear extracts; and after addition of HCK promoter sequence in LPS-stimulated BCWM.1 cell nuclear extract. (C) PROMO-weighted consensus TF-binding sites for TFs predicted to bind to the HCK promoter.