Deletion of STAT3, AP-1, and NF-kB consensus DNA motifs reduces HCK promoter activity and their binding to HCK promoter in MYD88-mutated WM and ABC-DLBCL cells. HCK promoter activity was assessed following deletion of AP-1, STAT3, and NF-kB binding sites in TNF-α–stimulated HEK-293 cells (A), and MYD88-mutated BCWM.1 and TMD-8 cells (B). (C) The binding of biotin-labeled HCK promoter to the corresponding TFs NF-kB, STAT3, and AP-1 was also reduced by indicated mutants in pull-down assays. **P < .01; ***P < .001.