Differential expression analysis reveals gene transcripts recurrently enriched in either malignant or normal B cells from the same biopsy. (A) Genes with strong and recurrent patterns of enrichment are at the top and bottom of the heat map. The heat map is restricted to genes differentially expressed at ≥ 60% AUC power. (B) Overlay of class II MHC gene expression (HLA-DR) on the tSNE map shows strong differences between normal and malignant B cells for all samples, albeit in variable directions. (C) Tumor-specific expression of HLA-DR predicted from scRNA-Seq data (top) was confirmed by flow cytometry (bottom).