Higher serum MCP-1 and IL-7 concentrations in patients with aggressive NHL who received more intensive lymphodepletion. Serum day 0 MCP-1 (A) and day 0 IL-7 (B) increase after lymphodepletion. Serum cytokine concentrations before lymphodepletion and on day 0 before CAR T-cell infusion for each patient are connected. P values comparing cytokine concentrations before lymphodepletion and on day 0 before CAR T-cell infusion were calculated by using the Wilcoxon rank-sum test. (C) The change in serum MCP-1 concentration between the pre-lymphodepletion and day 0 time points in patients who received high-intensity Cy/Flu lymphodepletion (red) and in those who received low-intensity Cy/Flu lymphodepletion (blue) are shown. Serum day 0 MCP-1 (D) and peak IL-7 (E) concentrations in patients who received high-intensity Cy/Flu lymphodepletion (red) and in those who received low-intensity Cy/Flu lymphodepletion (blue). Each point in panels C-E represents data from a single patient. Box and whisker plots show the median (bar) and interquartile range (box). Adjusted P values were calculated by using the Wilcoxon rank-sum test. (F) Serum day 0 MCP-1 and peak IL-7 concentrations according to lymphodepletion intensity (Spearman correlation r = 0.52; P = .0001). Each point represents data from a single patient.