Higher CAR T-cell counts in patients with aggressive NHL who received high-intensity lymphodepletion or had high serum MCP-1 concentration. CAR T-cell copies/μg of DNA by qPCR in patients who received high-intensity Cy/Flu lymphodepletion (red) and in those who received low-intensity Cy/Flu lymphodepletion (blue; P = .0005 for the difference in the peak of CAR T cells between the groups) (A); and in patients with serum day 0 MCP-1 concentration above the median (red) or below or equal to the median (blue; P = .03 for the difference in the peak of CAR T cells between the groups) (B). Each thin line in panels A-B represents a single patient; bold lines represent the averaged data using local polynomial regression curve fitting approximation, with the standard error in gray. Adjusted P values were calculated by using the Wilcoxon rank-sum test. (C) Association of day 28 CAR T-cell counts by qPCR (log₁₀ copies/μg DNA) and the hazard of PFS event using a restricted cubic spline with 3 knots. Tick marks represent data from individual patients.