Identification of IRF8-expressing LMPPs. (A) Flow cytometry analysis of HSCs and early progenitors in IRF8-GFP mice. Data are representative of 5 independent experiments. (B) Hierarchical clustering of scRNA-seq data in WT LMPPs. Transcription factor genes upregulated or downregulated more than fivefold in LMPPs compared with CDPs were selected. Red and blue gene symbols denote upregulated and downregulated genes in CDPs, respectively. The Irf8 transcript-expressing cluster is enclosed by a green rectangle. (C) Hierarchical clustering of microarray transcriptome data in hematopoietic populations. Mo, monocyte; neu, neutrophil.