IRF8⁺ LMPPs are derived from IRF8⁻ LMPPs. Flow cytometric analysis of bone marrow progenitors 3 days after intravenous transplantation of LMPP subpopulations. A total of 30 000 LMPPs were transplanted into a nonirradiated Ly5.1 mouse, and donor-derived cells were analyzed. Representative FACS plots of donor-derived LMPP subpopulations (A), MDPs (B), CDPs (B), and pre-cDCs (C) from 3 independent experiments. (D) Absolute cell numbers (per lower mouse limbs) of the indicated progeny cells derived from transplanted LMPPs are calculated. Values in the bar graph are the mean ± standard deviation from 3 independent experiments. *P < .05, **P < .01, ***P < .001 (Student t test).