ATAC-seq analysis of IRF8⁺ LMPPs. ATAC-seq analyses were performed in IRF8⁻ and IRF8⁺ LMPPs isolated from IRF8-GFP mice. Distal ATAC-seq peaks within H3K4me1-marked regions were selected. (A) A Venn diagram showing the overlap of ATAC-seq peaks between LMPP subpopulations. (B) Genome browser images of ATAC-seq data at the Jak2 and Irf8 gene loci. Blue rectangles indicate IRF8⁻ LMPP (top) and IRF8⁺ LMPP (bottom) specific peaks. The orange rectangle indicates a previously reported Irf8 enhancer region.²⁴  (C) De novo motif analysis of ATAC-seq peak regions specific for each subpopulation and those common to 2 subpopulations. (D) GSEA comparing hematopoietic populations for genes associated with IRF8⁺ LMPP-specific ATAC-seq peaks. (E) Heat map showing NES of GSEA. NES, normalized enrichment score.