IRF8 governs the chromatin accessibility of DC lineage enhancers in IRF8⁺ LMPPs. ATAC-seq analyses were performed in WT and Irf8^−/− LMPPs. IRF8⁺ LMPP-specific 1434 ATAC-seq peak regions detected in WT LMPPs were analyzed. (A, left) ATAC-seq tag-densities of the peak regions in WT and Irf8^−/− LMPPs are shown in violin plots. Student t test was performed for statistical significance. (A, right) The presence or absence of the 1434 ATAC-seq peaks were judged in Irf8^−/− LMPPs. (B) De novo motif analysis of IRF8⁺ LMPP-specific peaks lost in Irf8^−/− LMPPs (ie, 846 peaks). (C) Genome browser image of ATAC-seq data at the Ifi44 gene locus. Blue rectangle indicates an IRF8-dependent IRF8⁺ LMPP-specific peak. (D, left) Overlap between LMPP subpopulation-specific ATAC-seq peaks and IRF8 ChIP-seq peaks in MDPs. (D, right) Heat maps of IRF8 binding in MDPs at the LMPP subpopulation-specific ATAC-seq peak regions. Each horizontal line represents the density of IRF8 ChIP-seq tags in the 4-kb region centered on the ATAC-seq peak summit.