INKA1-OE induces quiescence in primitive 8227 AML cells. (A) Shown are sorted 8227 AML BFP⁺CD34⁺CD38⁻ (+/−) and BFP⁺CD34⁺CD38⁺BFP⁺(+/+) that were stained for intracellular Ki67 expression and DNA content (Hoechst) staining. (B) Cell cycle distribution of transduced CD34⁺CD38⁻BFP⁺ cells (n = 3). (C) Transduced 8227 AML cells were labeled with CellTrace Far Red and analyzed 5 days later by flow cytometry (n = 3). (D) Transduced cells were incubated with BrdU and harvested after 20 hours for intracellular detection of BrdU incorporation (n = 3). Student t test *P < .05; **P < .01; ***P < .001.