ASXL1 schematic structure and localization of exon 12 mutations. Regions encoding the SRC1-binding domain and lysine-specific demethylase-1 (LSD1)-binding domain are indicated. Each single mutation is shown as: blue diamond, nonsense; red triangle, indel; green dot, missense mutations (A). Overall survival (OS) for patients stratified according to the presence of G646Wfs*12 variant, other ASXL1 mutations, and a wild-type (WT) genotype is shown as Kaplan-Meier survival curves for all patients of the cohort (n = 333; B), patients with prefibrotic PMF (n = 139; C), and overt PMF (n = 194; D). The leukemia-free survival (LFS) for all patients of the cohort is shown in panel E. CI, confidence interval; HR, hazard ratio; mut, mutation; NR, not reached; yr/yrs, year/years.