ARIEL promotes the ARID5B enhancer-promoter interaction. (A) Jurkat cells were transduced with either a control shRNA (shGFP) or an shRNA targeting ARIEL (sh3) by lentivirus infection. Genomic DNA samples were harvested on day 3 of the shRNA transduction and subjected to a 4C assay. A heatmap showing the interactions from the viewpoint (ARID5B −135-kb enhancer locus) of the neighboring genomic regions before and after the knockdown of ARIEL. Color codes represent the quantitative scale (q-value). A red arrowhead indicates the ARID5B promoter. (B) ChIRP assay was performed using 2 different sets of antisense probes (“set 1” and “set 2”) that detect ARIEL or control probes (anti-Lacz). The amount of genomic DNA in the ChIP and input samples was measured by performing qPCR using specific primers targeting the ARID5B −135-kb enhancer locus or the ARID5B promoter region. The neighboring locus (CDK1 promoter) was used as a negative control region. The fold-enrichments are shown as the mean plus or minus SD of duplicate experiments. (C) Proposed model.