Progenitor phenotypes exhibit distinct but overlapping molecular profiles. (A) t-SNE distributions determined from the levels of different surface and intracellular markers for each lin^–CD34⁺ CB phenotype analyzed. Increasing cell densities are indicated by increasing color intensities. Black contours delineate the 75th quantile of the overall density distribution. Plots for the CD34⁺CD38^– subpopulations are shown on the left, and for the CD34⁺CD38⁺ subpopulations, on the right. (B) Relative levels of different TFs (left) and signaling proteins (right) across the t-SNE distributions of all CD34⁺ cells analyzed. (C) t-SNE distributions for alternative phenotypes are shown as in (A). The far-right panel shows a hierarchical clustering of all phenotypically defined populations (8 conventional phenotypes plus 3 new phenotypes) based on pairwise differences in their density distributions. HSC, hematopoietic stem cells.