Lineage information is contained in multiple protein parameters, including signaling states. (A) t-SNE distributions obtained using all 40 analyzed parameters; TFs only (GATA3, PAX5, PU.1, TAL1, CEBPα, and GATA1); surface markers only (CD45RA, CD71, CD45, CD114, CD123, CD34, CD33, CD49f, CD10, CD135, CD38, CD90, HLADR, and CD133); intracellular markers only (pSHP2, GATA3, pCRKL, pSrc, pACC, Cyclin B1, PAX5, PU.1, pSTAT5, pAKT, pSTAT1, pSMAD2/3, pP38, pSTAT3, pMAPKAPK2, IκBα, pCREB, active β-catenin, pERK1/2, Ki67, pSykZap70, TAL1, CEBPα, pS6, GATA1, and peEF2); active signaling intermediates (pSHP2, pCRKL, pSrc, pACC, pSTAT5, pAKT, pSTAT1, pSMAD2/3, pP38, pSTAT3, pMAPKAPK2, IκBα, pCREB, active β-catenin, pERK1/2, pSyk/Zap70, pS6, and peEF2), or surface markers together with TFs. (B) Hierarchical clustering of phenotypically defined populations based on pairwise differences in each of their t-SNE distributions from (A).