NETs are reduced by NAC in MPN patients. (A) Representative immunofluorescence images of NETs from normal and MPN individuals. H3cit is shown in green, and DAPI-stained DNA is shown in blue. Arrows represent H3cit+ NETs. Bars represent 50 μm. Original magnification ×40. (B-C) Quantification of NETs from normal individuals (B) or patients with MPN (C) upon stimulation with 10 nM PMA and PMA + NAC; **P < .01, ****P < .0001 1-way ANOVA. (D) Representative time course of extracellular DNA via RFUs as measured by the nucleic acid dye SYTOX. (E) Quantification of RFUs normalized to unstimulated normal neutrophils. The inhibitor diphenyleneiodonium (DPI) was used as a positive control for NET inhibition. Each point represents an individual; *P < .05, ***P < .001, ****P < .0001, Student t test. Error bars represent SEM. Shown are representative data from 3 biological replicates.