Expression of results of both green and red JC-1 fluorescence intensities.

The function of Pgp was established with blast cells selected by CD34 antibody or by physical characteristics. Because delta psi changes that occur during apoptosis are manifested by a loss of red fluorescence and because CsA can modify delta psi changes, we initially analyzed the red fluorescence intensity without CsA. The results are very similar if the results of red fluorescence intensity are presented as (1) a percentage of positive cells with CsA or without CsA, (2) absolute red fluorescence intensity, or (3) ratio of red fluorescence with or without CsA.