Fig. 2.
Comparison between 2 Pgp functional assays (Rh123 and JC-1 [green fluorescence] with and without CsA).
We and others, in precedent studies, have used the threshold of positivity of 0.2 in the Pgp activity detection assay using Rh123. The threshold of positivity for JC-1 assay (green fluorescence) was 0.62 when compared with the threshold of positivity of Rh123 functional assay.