Comparison between green and red fluorescences of JC-1 probe.

In a previous study8 using JC-1 in cell lines, all samples positive in green fluorescence were positive in red fluorescence and no patients were positive in red fluorescence and negative in green fluorescence. Therefore, the threshold of positivity in red fluorescence was determined at 50%. Because δψ changes that occur during apoptosis are manifested also by a loss of red fluorescence and CsA can modify δψ changes, we initially analyzed the results of red fluorescence intensity without CsA. But the results are very similar if the results of red fluorescence intensity are presented as (1) percentage of positive cells with CsA or percentage of positive cells, (2) absolute red fluorescence intensity, or (3) the ratio of red fluorescence with or without CsA.