SDS-PAGE of LL2-onconase conjugate under reducing and nonreducing conditions.

LL2-onconase was prepared as described in “Materials and methods” and analyzed on a 4% to 20% polyacrylamide gel. Lanes 1-4, samples were reduced by boiling for 5 minutes in SDS sample buffer containing 2-mercaptoethanol. Lanes 1 and 2, 6 and 3 μg LL2-onconase, respectively; lane 3, 1 μg LL2; lane 4, 1 μg onconase. Lanes 5 and 6 were under nonreducing conditions. Lane 5, 3 μg LL2-onconase; lane 6, 1 μg LL2 antibody. The gel was stained with Coomassie Blue.