American Society of Hematology

Fig. 1.

$Fig. 1. Percoll Larex continuous density gradient of the 5 types of transgenic mice. / The lightest cells (at the top of the gradient) are predominantly reticulocytes and the densest cells (at the bottom of the gradient) are enriched in ISCs. The MCHC found at each depth in the tube is displayed on the left hand side of the gradients. The gradient has physiologic pH and osmolarity and is performed at 37°C. From left to right: density-marker beads, C57BL, NY1KO γL, NY1KO γM, NY1KO γH, BERK, and BERK γM. Note the highest density (MCHC) cells in the NY1KO γM mouse and the lowest density (MCHC) cells in the BERK mouse. The horizontal black line is included for reference: note in particular that about 40% of the NY1KO γL red cells have a density greater than that indicated by the line, more than 90% of NY1KO γM red cells have a density greater than that indicated by the line, and that less than 10% of the BERK red cells have a density greater than that indicated by the line. All 3 NY1KO mice have a fraction of cells lighter than the C57 mouse that are predominantly reticulocytes and appear in proportion to the percentage reticulocytes: 63% reticulocytes for NY1KO γL (less than 3% γ), 30% reticulocytes for the NY1KO γM (20% γ), and 13% for the NY1KO γH (40% γ). The percentage high-density cells is greater in the NY1KO γM mouse with 20% γ. In contrast, the BERK mouse12 has very few red cells with a density equal to or greater than the C57 red cells. When γM is introduced into the BERK mouse, red cell density increases resulting in more cells with a nearly normal MCHC; note that, in contrast to the BERK mouse, more than 90% of the red cells have a density greater than that indicated by the black line. Separation of the gradient of the BERK γM mouse into a top and bottom fraction shows that there is a higher percentage γ in the bottom fraction.$

Percoll Larex continuous density gradient of the 5 types of transgenic mice.

The lightest cells (at the top of the gradient) are predominantly reticulocytes and the densest cells (at the bottom of the gradient) are enriched in ISCs. The MCHC found at each depth in the tube is displayed on the left hand side of the gradients. The gradient has physiologic pH and osmolarity and is performed at 37°C. From left to right: density-marker beads, C57BL, NY1KO γL, NY1KO γM, NY1KO γH, BERK, and BERK γM. Note the highest density (MCHC) cells in the NY1KO γM mouse and the lowest density (MCHC) cells in the BERK mouse. The horizontal black line is included for reference: note in particular that about 40% of the NY1KO γL red cells have a density greater than that indicated by the line, more than 90% of NY1KO γM red cells have a density greater than that indicated by the line, and that less than 10% of the BERK red cells have a density greater than that indicated by the line. All 3 NY1KO mice have a fraction of cells lighter than the C57 mouse that are predominantly reticulocytes and appear in proportion to the percentage reticulocytes: 63% reticulocytes for NY1KO γL (less than 3% γ), 30% reticulocytes for the NY1KO γM (20% γ), and 13% for the NY1KO γH (40% γ). The percentage high-density cells is greater in the NY1KO γM mouse with 20% γ. In contrast, the BERK mouse12 has very few red cells with a density equal to or greater than the C57 red cells. When γM is introduced into the BERK mouse, red cell density increases resulting in more cells with a nearly normal MCHC; note that, in contrast to the BERK mouse, more than 90% of the red cells have a density greater than that indicated by the black line. Separation of the gradient of the BERK γM mouse into a top and bottom fraction shows that there is a higher percentage γ in the bottom fraction.

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