American Society of Hematology

Fig. 5.

$Fig. 5. Binding of soluble muIfnar-2 to type I IFN. / (A) Table showing the specific binding of 32P-muIFNα1 to 5 ng/μL and 10 ng/μL recombinant muIfnar-2 ECD. Data are expressed as the mean ± SD cpm. (B) Histogram showing the elution profile of total 32P-muIFNα1 bound to high-molecular weight complexes in serum. The mean cpm of fractions 2 to 4 were used to determine total binding (mean = 80 403 cpm), specific binding (mean = 38 440 cpm) and nonspecific binding (mean = 41 963 cpm). VO indicates void volume.$

Binding of soluble muIfnar-2 to type I IFN.

(A) Table showing the specific binding of ³²P-muIFNα1 to 5 ng/μL and 10 ng/μL recombinant muIfnar-2 ECD. Data are expressed as the mean ± SD cpm. (B) Histogram showing the elution profile of total ³²P-muIFNα1 bound to high-molecular weight complexes in serum. The mean cpm of fractions 2 to 4 were used to determine total binding (mean = 80 403 cpm), specific binding (mean = 38 440 cpm) and nonspecific binding (mean = 41 963 cpm). V_O indicates void volume.

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